Biorad Parameter Generator

This excel file will calculated the ideal iris diameter for an airy disk of 1, the nyquist sampling rates, and the backprojected pinhole radius for previously calculated airy disk diameter. All that needs to be entered is the magnification and numerical aperture (N.A.) of the objective, the excitation wavelength, and refractive index (R.I.) of the lens. It can also calculate the backprojected pinhole radius for other iris diameters. The Nyquist sampling rates are in NANOMETERS, convert them to microns for use in the Biorad software.

This information is only valid for a BIORAD machine.