Calibrations
Submitted by staff on Fri, 2005-06-03 14:04
| Confocal 1000 Scale Bar Chart (chart assumes image size was not changed from default of 512x512, also that a zoom of 1 was used) | Confocal 1024 Scale Bar Chart (chart assumes image size was not changed from default of 512x512, also that a zoom of 1 was used) | ||
| Use the following table to create a scale bar: | Use the following table to create a scale bar: | ||
| Objective | Scale Bar | Objective | Scale Bar |
| 4x | 60 pixels = 250 microns | 4x | 51 pixels = 250 microns |
| 10x | 60 pixels = 100 microns | 10x | 51 pixels = 100 microns |
| 20x | 60 pixels = 50 microns | 20x | 51 pixels = 50 microns |
| 40x | 60 pixels = 25 microns | 40x | 51 pixels = 25 microns |
| 60x | 75 pixels = 20 microns | 60x | 64 pixels = 20 microns |
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| FluoView 300 Custom MultiPhoton Confocal Scale Bar Chart (chart assumes image size was not changed from default of 512x512, also that a zoom of 1 was used) | FluoView 1000 Confocal Scale Bar Chart (chart assumes image size was not changed from default of 512x512, also that a zoom of 1 was used) | ||
| Use the following table to create a scale bar: | Use the following table to create a scale bar: | ||
| Objective | Scale Bar | Objective | Scale Bar |
| 4x | 21.4 pixels = 100 microns | ||
| 10x dry | 71 pixels = 100 microns | 10x | 53.4 pixels = 100 microns |
| 20x water | 145 pixels = 100 microns | 20x | 107 pixels = 100 microns |
| 40x water | 290 pixels = 100 microns | 40x | 106 pixels = 50 microns |
| 60x oil | 434 pixels = 100 microns | 60x | 160 pixels = 50 microns |
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| Factoring Scales for Metamorph I (Olympus B-Max 06 Microscope - chart assumes image size was not changed from default of 512x512, also that a zoom of 1 was used) | Factoring Scales for Metamorph I (Olympus B-Max 06 Microscope, Scion Camera - chart assumes image size was not changed from default of 512x512, also that a zoom of 1 was used) | ||
| Use the following table to create a scale bar: | Use the following table to create a scale bar: | ||
| Objective | Scale Bar | Objective | Scale Bar |
| 4x | 440 pixels = 100 microns | ||
| 10x | 60 pixels = 100 microns | 10x | 111 pixels = 100 microns |
| 20x | 60 pixels = 50 microns | 20x | 225 pixels = 100 microns |
| 40x | 60 pixels = 25 microns | 40x | 450 pixels = 100 microns |
| 60x | 75 pixels = 20 microns | 60x water | 682 pixels = 100 microns |
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| Factoring Scales for Zeiss (Zeiss Microscope, SPOT camera - chart assumes image size was not changed from default of 512x512, also that a zoom of 1 was used) | Inverted and PixCell LCM (Olympus Microscope, DVC camera - chart assumes image size was not changed from default of 512x512, also that a zoom of 1 was used) | ||
| Use the following table to create a scale bar: | Use the following table to create a scale bar: | ||
| Objective | Scale Bar | Objective | Scale Bar |
| 2.5x | 25 pixels = 100 microns | 2x | 31 pixels = 100 microns |
| 4x | 38 pixels = 100 microns | 4x | 62 pixels = 100 microns |
| 10x | 95 pixels = 100 microns | 10x | 155 pixels = 100 microns |
| 20x | 190 pixels = 100 microns | 20x | 308 pixels = 100 microns |
| 40x | 190 pixels = 50 microns | 40x | 306 pixels = 50 microns |
| 63x | 119 pixels = 20 microns | 60x | 187 pixels = 20 microns |
